Journal: Scientific Reports

Article Title: Genetic editing of primary human dorsal root ganglion neurons using CRISPR-Cas9

doi: 10.1038/s41598-025-91153-2

Figure Lengend Snippet: Lipofectamine 3000 reagent protocol. The hDRG cells were transfected according to these optimized specifications.

Article Snippet: BrainPhys ® media (Stemcell technologies, cat. no. 05790), 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15070063), 1% GlutaMAX ® (United States Biological, cat. no. 235242), 2% NeuroCultTM SM1 (Stemcell technologies, cat. no. 05711), 1% N-2 Supplement (Thermo Scientific, cat. no. 17502048), 2% HyCloneTM Fetal Bovine Serum (ThermoFisher Scientific SH3008803IR), 1: 1000 FrdU, 10 ng/mL Human β nerve growth factor - (Cell Signaling Technology, Cat# 5221SC).

Techniques: Transfection

Journal: Cell Systems

Article Title: Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis

doi: 10.1016/j.cels.2018.08.011

Figure Lengend Snippet:

Article Snippet: After 4 dpi, half of the mTeSR 1 media was replaced with supplemented BrainPhys media (05790, StemCell Technologies), replaced daily until 7 dpi and further once per week.

Techniques: Recombinant, Luciferase, cDNA Synthesis, Plasmid Preparation, Software